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The tender-clipped sequences that overhang the breakpoint are collapsed right into a consensus sequence utilizing a multiple sequence alignment (MSA) presented inside the seqAn library [27]. Wham applies three filters to your consensus sequences. Breakpoints usually are not claimed in circumstances where by consensus sequences are shorter than ten … Read More


Wham is suitable for paired-close Illumina libraries with normal insert sizes (~300bp-500bp). Wham integrates mate-pair mapping, split examine mapping, soft-clipping, substitute alignment and consensus sequence primarily based proof to predict SV breakpoints with one-nucleotide accuracy. Wham generates a put together pileup (catalog of reads maskin… Read More


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